Taxonomy: Class Blastocystea
Animal: Blastocystis hominis 8 18.jpg
Sites: Gut
Blastocystis hominis - Trichrome stain. Vacuolar forms (has a central vacuole with 1 - 4 nuclei and 5 - 20 microns wide) and amoeboid form (these forms inhabit the colon along with these small avacuolar forms but may be overlooked because of their small size, 5 microns or because they degenerate after passage). The vacuolar form is found in culture or passed from the colon and was at first thought to be the typical and only forms of B.hominis but are now known not to be the forms found in the colon. Unless the specimen is examined fresh or fixed with PVA or formalin immediately on passing, the in vivo forms ( avacuolar, amoeboid and multi- vacuolar forms) will have deteriorated and only the CV or granular forms will be seen in wet preparations, cysts will be missed if a stained smear is not examined due to their small size - 5 microns.

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